Effect of Ultrasonic Irradiation on Aggregate and Triple-helix Structure of Collagen in Acetic Acid Solution

Circular dichroism(CD) is a routine method to figure the triple-helix structure of collagen.In the present study,by comparing the changes of molar ellipticity(Em) and optical rotation(α) of collagen in acetic acid solution during its thermal denaturation process,it was found that the change of optical rotation could show the denaturation of collagen solution and the status of collagen aggregate and collagen triple-helix.Based on that,the sonication of collagen in acetic acid solution at 40 kHz,120 W by monitoring the time dependence of optical rotation of collagen showed that the cavitation effect of ultrasonic irradiation was not able to destroy the triple-helix structure of collagen,but make collagen aggregate loosen to some extend.The further measurement by ultra-sensitive different scanning calorimetry(US-DSC) also proved the same results.