A Study on the Mechanisms of Inhibiting Poliferous Effect on Human Breast Cancer

Objective To study the mechanisms of inhibiting proliferous effect of human breast cancer cell line treated with Genistein in vitro.Methods Two human breast cancer cell lines(MCF-7 and MDA-MB-231) were cultured in vitro,proliferation of them was measured with MTT method,cell morphology was observed with Giemsastain,cell cycle and apoptosisrate were measured with flow cytometry and apoptosis was detected in situ.Results Genistein markedly inhibited proliferation of MCF-7 and MDA-MB-231 cell line.Typical image of apoptosis was observed in MCF-7 cell line treated with Genistein in Giemsastain: cell condensation,blebs formation on cell surface and pieces of condensed chromatin were scattered along nuclear envelope.The results detected with flow cytometry showed G1 subpeak before G1 phase peak(apoptosis peak).Apoptosis rate increased withtime of Genistein treatment.However,MDA-MB-231 cell line treated with Genistein did not show apoptosis and apoptosis peak,and quantity of cells at G2～Mphase obviously increased.Conclusion Genistein inhibites proliferation of human breast cancer cellline in vitro through inducing apoptosis or arresting cell at G～M phase.Genistein can induce MCF27 cells apoptosis and it may be one of the mechanisms for theinhibitory effect of genistein in human cancer cells.