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2013•Journal of Henan UniversityRequires access

Identification of the Strain G7 and Its Biocontrol Effects against the Seedlings Damping-off of Cucumber

Gang Wang

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Abstract

Biological control efficacy of strain G7 against the seedlings damping-off incited by Rhizoctonia solani of cucumber was evaluated in pots test.The results showed that G7 could control the harm efficiently and the control efficacy were 82%~96%.G7 was identified as Bacillus subtilis by the analysis of its morphology,biochemical characteristics and 16s rRNA gene sequence.The strain has enzymatic activities of chitosanase,protease and glucanase.The hyphen growth inhibition of G7 against R.solani was also observed by means of dual-culture test.

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Biological control efficacy of strain G7 against the seedlings damping-off incited by Rhizoctonia solani of cucumber was evaluated in pots test.The results showed that G7 could control the harm efficiently and the control efficacy were 82%~96%.G7 was identified as Bacillus subtilis by the analysis of its morphology,biochemical characteristics and 16s rRNA gene sequence.The strain has enzymatic activities of chitosanase,protease and glucanase.The hyphen growth inhibition of G7 against R.solani was also observed by means of dual-culture test.

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Available abstract

Biological control efficacy of strain G7 against the seedlings damping-off incited by Rhizoctonia solani of cucumber was evaluated in pots test.The results showed that G7 could control the harm efficiently and the control efficacy were 82%~96%.G7 was identified as Bacillus subtilis by the analysis of its morphology,biochemical characteristics and 16s rRNA gene sequence.The strain has enzymatic activities of chitosanase,protease and glucanase.The hyphen growth inhibition of G7 against R.solani was also observed by means of dual-culture test.

Key concepts: Chitosanase, Bacillus subtilis, Rhizoctonia solani, Biology, Damping off, Strain (injury), 16S ribosomal RNA, Biological pest control

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