Immunization Effect Test for Recombinant Avian Influenza Virus H5 Subtype Bivalent Inactivated Vaccine(H5N1,Re-4 + Re-5)in Pigeon

640 pigeons of above one month old were randomly selected and grouped,then isolation tests were made respectively inoculating 0.2,0.3,0.4,0.5,1.0,1.5 and 2.0 mL recombinant avian influenza virus H5 subtype bivalent inactivated vaccine(H5N1,Re-4+Re-5).In the third week after the first immunization,the second same dose injection for half pigeon quantity of vaccine test groups with 0.2,0.3,0.4 and 0.5 mL was done.Blood samples were collected periodically,and avian influenza antibodies by hemagglutination-inhibition test method were detected.The results showed that Re-4 with a higher antibody titer was better than Re-5 for the first immunization;their effects were similar for the second immunization and the whole effect of the second immunization was better than the first immunization.In order to form a good immune effect,vaccine need to be inoculated for one-time immunization at least 0.4 mL and for two-time immunization at least 0.3 mL.