2007•Di-san junyi daxue xuebaoRequires access
Effects of heat shock preconditioning on expressions of HSP60,HSP70 and iNOS in gastric mucosa of burned rats
Hongyan Zhang, Lu Nonghua, Yong Xie, Guanghua Guo, Jianhua Zhan, Chen Jiang
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Abstract
Objective To observe the influence of heat shock preconditioning on the expressions of heat shock protein(HSP)60,70 and induced nitric oxidase synthase(iNOS)in gastric mucosa of severely burned rats and investigate the protective mechanism of burn-induced acute gastric mucosal lesion.Methods Ninety-six Wistar rats were divided into two groups:burn(B)and heat shock preconditioning before burn(HB).The extent of gastric mucosal lesions(UI)was evaluated grossly and histologically.HSP70 mRNA expression was determined by RT-PCR.Immunohistochemical method was used to analyze the protein expressions of iNOS,HSP60 and HSP70.Results Rats in B group were induced conspicuous gastric mucosal lesions,while UI in HB group was significantly lower.The expressions of HSP70 and HSP60 were significantly increased and the expression of iNOS was obviously decreased in HB group as compared with B group.Conclusion Heat shock preconditioning protects against acute gastric mucosal lesion caused by burn through enhancing the expressions of induced HSPs and reducing the iNOS expression.