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Development and application of Real-time PCR for the detection of Toxoplasma gondii

Jinlin Zhou

Open publisher page 1 citations

Abstract

The specific primers and probes were designed targeting the 529 bp fragment of Toxoplasma gondii,and thus a TaqMan Real-time PCR was developed.360 clinical samples were detected using the Real-time PCR,and the detection data were compared with conventional PCR and loop-mediated isothermal amplification(LAMP) techniques.We found that the positive rate by Real-time PCR(11.11%) was higher than LAMP(7.5%) and conventional PCR(3.61%).The detection results by Real-time PCR for 750 clinical samples,which were collected from four kinds of animals in different regions,showed that T.gondii infection could be detected in the blood samples of different species of animals.The infection rate was found highest in sheep(17.8%),followed by pigs(4.22%),cattle(1.98%) and dogs(1.37%).

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What this paper is about

The specific primers and probes were designed targeting the 529 bp fragment of Toxoplasma gondii,and thus a TaqMan Real-time PCR was developed.360 clinical samples were detected using the Real-time PCR,and the detection data were compared with conventional PCR and loop-mediated isothermal amplification(LAMP) techniques.We found that the positive rate by Real-time PCR(11.11%) was higher than LAMP(7.5%) and conventional PCR(3.61%).The detection results by Real-time PCR for 750 clinical samples,which were collected from four kinds of animals in different regions,showed that T.gondii infection could be detected in the blood samples of different species of animals.The infection rate was found highest in sheep(17.8%),followed by pigs(4.22%),cattle(1.98%) and dogs(1.37%).

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Available abstract

The specific primers and probes were designed targeting the 529 bp fragment of Toxoplasma gondii,and thus a TaqMan Real-time PCR was developed.360 clinical samples were detected using the Real-time PCR,and the detection data were compared with conventional PCR and loop-mediated isothermal amplification(LAMP) techniques.We found that the positive rate by Real-time PCR(11.11%) was higher than LAMP(7.5%) and conventional PCR(3.61%).The detection results by Real-time PCR for 750 clinical samples,which were collected from four kinds of animals in different regions,showed that T.gondii infection could be detected in the blood samples of different species of animals.The infection rate was found highest in sheep(17.8%),followed by pigs(4.22%),cattle(1.98%) and dogs(1.37%).

Key concepts: TaqMan, Toxoplasma gondii, Real-time polymerase chain reaction, Loop-mediated isothermal amplification, Biology, Polymerase chain reaction, Molecular biology, Virology

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Development and application of Real-time PCR for the detection of Toxoplasma gondii — Research Paper | ScholarLens