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2000•Journal of Suzhou Institute of Silk Textile TechnologyRequires access

STUDY ON THE PROPERTIES OF BOMBYX MORI NUCLEAR POLYHEDROSIS VIRUSWITH HETEROGENEOUS POLYHEDRIN

Ping Ji

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Abstract

The polyhedrin gene and its promoter of Autographa californica multicapsid nuclear polyhedrosis virus(AcMNPV) was separated by PCR.Then cloned it into transfer vector pBacPAK(API4) and obtained recombinant transfer vector pBacOAc(API4).Contransfected in BmN cells with CrBK genome which has been deleted the polyhedrin gene of Bombyx mori nuclear polyhedrosis virus.And a recombinant BmNPV(OAc-API4) was obtained,which was able to produce AcMNPV polyhedrin protein and expressed the Arrowhead proteinase inhibitor.The replication of the recombinant virus in silkworm cells and larvae showed that the budded virus could infect BmN cells and injected silkworm larvae,while the per os inoculation with its occluded virus couldn′t infect the larvae.It was indicated that the per os inoculation of NPV was concerned with the type of polyhedrin proteins.

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What this paper is about

The polyhedrin gene and its promoter of Autographa californica multicapsid nuclear polyhedrosis virus(AcMNPV) was separated by PCR.Then cloned it into transfer vector pBacPAK(API4) and obtained recombinant transfer vector pBacOAc(API4).Contransfected in BmN cells with CrBK genome which has been deleted the polyhedrin gene of Bombyx mori nuclear polyhedrosis virus.And a recombinant BmNPV(OAc-API4) was obtained,which was able to produce AcMNPV polyhedrin protein and expressed the Arrowhead proteinase inhibitor.The replication of the recombinant virus in silkworm cells and larvae showed that the budded virus could infect BmN cells and injected silkworm larvae,while the per os inoculation with its occluded virus couldn′t infect the larvae.It was indicated that the per os inoculation of NPV was concerned with the type of polyhedrin proteins.

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Available abstract

The polyhedrin gene and its promoter of Autographa californica multicapsid nuclear polyhedrosis virus(AcMNPV) was separated by PCR.Then cloned it into transfer vector pBacPAK(API4) and obtained recombinant transfer vector pBacOAc(API4).Contransfected in BmN cells with CrBK genome which has been deleted the polyhedrin gene of Bombyx mori nuclear polyhedrosis virus.And a recombinant BmNPV(OAc-API4) was obtained,which was able to produce AcMNPV polyhedrin protein and expressed the Arrowhead proteinase inhibitor.The replication of the recombinant virus in silkworm cells and larvae showed that the budded virus could infect BmN cells and injected silkworm larvae,while the per os inoculation with its occluded virus couldn′t infect the larvae.It was indicated that the per os inoculation of NPV was concerned with the type of polyhedrin proteins.

Key concepts: Polyhedrin, Nuclear Polyhedrosis Virus, Autographa californica, Bombyx mori, Virology, Recombinant DNA, Biology, Recombinant virus

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STUDY ON THE PROPERTIES OF BOMBYX MORI NUCLEAR POLYHEDROSIS VIRUSWITH HETEROGENEOUS POLYHEDRIN — Research Paper | ScholarLens