Studies of CD258-Fc gene transfection into human bladder transitional cell carcinoma T24 cell lines inhibition proliferation in vivo and in vitro

[Objective] To investigate the inhibition proliferation effect of CD258-Fc on human bladder transitional cell carcinoma T24 cell lines in vitro and in vitro and the inhibitory rate in nude mice model so as to discuss CD258-Fc gene transfection into T24 cell lines seeking a new way for gene therapy of carcinoma of human Bladder Transitional Cell Carcinoma (BTCC). [Methods] CD258-Fc expression vector or pc DNA 3.1 (+) blank vector was transfected into human BTCC T24 cell lines and normal T24 cells were used as normal contro1. The inducing apoptosis effects of CD258-Fc gene on cell growth was described by cell growth curve,MTT assay and flow cytometry (FCM) analysis. The expression of CD258 gene after transfection was detected by RT-PCR, Western blot assay for the protein. The nude mice model carried BTCC T24 cell lines were constructed, the nude mice were consecutively treated by CD258-Fc gene transfection, the inhibitory tumor rate was estimated. [Results] Expression of CD258-Fc gene strongly inhibited T24 cell proliferation in vitro. The growth of T24/CD258 was significantly lower than that of contro1. MTT test showed that the difference of cell viability between T24/CD258 and control cells was also significant (P 0.05). The expression of CD258 gene after transfection was highly detected by RT-PCR, the high expression of protein was detected by Western blot assay. FCM showed T24/CD258 cells on S phase was reduced greatly and apoptosis could be observed in some cells. In nude mice of BTCC model, the inhibitory rate in CD258-Fc gene transfection group without and with different IFN-γ concentrations got up to 48.66% and 60.98% (P 0.05), which differed remarkably (P =0.032). [Conclusions] The findings showed that the transfection of CD258-Fc gene induced cellular apoptosis in T24 cells and that the BTCC of the nude mice model were inhibited by CD258-Fc gene transfection which suggested that CD258-Fc gene transfection into T24 cells technique can be an effective means in the gene therapy of carcinoma of BTCC.