Clinical Value of Observation of Fluorescence Pattern of Antinuclear Antibody in Hep-2 Cell by Using Double Staining Method and Two Different Microscopes

Objective To evaluate the clinical value of fluorescence microscopy and confocal laser scanning microscope in detecting the fluorescence pattern of antinuclear antibody on Hep-2 cell using double fluorescent labeling. Methods The samples whose antinuclear antibody was positive were chosen for the study. These samples were incubated with FITC and DAPI. Finally,fluorescence microscopy and confocal laser scanning microscope were used to detect these samples respectively. Results The fluorescence of FITC and DAPI appeared on the position of antigen-antibody binding site and nucleus,respectively. It was easy to detect the fluorescence pattern after locating the nucleus by the fluorescent of DAPI. The fluorescence patterns of Speckled Pattern,Homogeneous Pattern,Centromere Pattern,Nuclear Dot Pattern,Karyotheca Pattern,Cytoplasmic granules type were observed. Conclusion The double fluorescent labeling with the confocal laser scanning microscope to detect the fluorescence pattern of antinuclear antibody on Hep-2 cell is the most helpful method for clinical detection.