Study on Antioxidant Activity of Flammulina velutipes Polysaccharide
Zhang Li-xi
Abstract
Zhang Li-xi
Abstract
In this paper,taken Flammulina velutipes as tested materials,the water-soluble polysaccharide and alkali-soluble polysaccharide from Flammulina velutipes were extracted with the method of boiling alcohol precipitation extraction. Antioxidant activity of Flammulina velutipes water-soluble polysaccharide and alkali-soluble polysaccharide were evaluated by the efficiency of scavenging the hydroxyl radical and superoxide anion radical. The results showed that the water-soluble polysaccharide and alkali-soluble polysaccharide of Flammulina velutipes had a certain capacity to scavenge hydroxyl radical and superoxide anion radical,and the efficiency of scavenging increased with the concentrations. When the water-soluble and the alkali-soluble polysaccharide concentration achieved 1. 6 mg / mL,the scavenging percentage of hydroxyl radical reached 32. 1 % and 57. 5 %,respectively; when the water-soluble or the alkali-soluble polysaccharide concentration achieved 2. 0 mg / mL,the scavenging percentage of superoxide anion radical reached 57. 4 % and 71. 3 %,respectively. Therefore,the Flammulina velutipes polysaccharide had a good role in anti-oxidation and anti-superoxide anion and had a great potential for development of anti-oxidation.
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In this paper,taken Flammulina velutipes as tested materials,the water-soluble polysaccharide and alkali-soluble polysaccharide from Flammulina velutipes were extracted with the method of boiling alcohol precipitation extraction. Antioxidant activity of Flammulina velutipes water-soluble polysaccharide and alkali-soluble polysaccharide were evaluated by the efficiency of scavenging the hydroxyl radical and superoxide anion radical. The results showed that the water-soluble polysaccharide and alkali-soluble polysaccharide of Flammulina velutipes had a certain capacity to scavenge hydroxyl radical and superoxide anion radical,and the efficiency of scavenging increased with the concentrations. When the water-soluble and the alkali-soluble polysaccharide concentration achieved 1. 6 mg / mL,the scavenging percentage of hydroxyl radical reached 32. 1 % and 57. 5 %,respectively; when the water-soluble or the alkali-soluble polysaccharide concentration achieved 2. 0 mg / mL,the scavenging percentage of superoxide anion radical reached 57. 4 % and 71. 3 %,respectively. Therefore,the Flammulina velutipes polysaccharide had a good role in anti-oxidation and anti-superoxide anion and had a great potential for development of anti-oxidation.
Key concepts: Flammulina, Chemistry, Polysaccharide, Hydroxyl radical, Superoxide, Radical, Food science, Organic chemistry