Primary culture of abomasum smooth muscle cells in newborn calf Comparison research on methods of tissue culture and collagenase digestion

Abomasum smooth muscle cells of newborn calf were isolated and cultured with tissue culture and collagenase digestion,and were indentified through immunocytochemistry and immunofluorescence technique,and then the results and the cell growth curves were compared.The cell growth curves based on the two ways were almost the same;the cell purity was more than 95%through tissue culture,and 90%through the collagenase digestion with the immunocytochemistry identification,which is almost the same to immunofluorescence results:96%through tissue culture and 89%through collagenase digestion respectively.Tissue culture method is easy to operate and economical, but takes a long time;collagenase digestion method is rapid,easy to obtain more cells,but very expensive,so we would like to use the tissue culture-in combination with collagenase digestion in practice.