Screening Differentially Expressed Genes in the Process of Retinoic Acid Inducing SH-SY5Y to Neuron by Oligonucleotide Microarray

Objective: To study the gene expression profile in the process of retinoic acid(RA) inducing SH-SY5Y to neuron by oligonucleotide microarray. Methods: Nerve associated genes were obtained from embryonic brain and different kinds of nervous tumors. Through GenBank, the mRNA sequence of the genes were acquired. The probes were designed and synthesized. Then oligonucleotide microarray were fabricated containing 218 genes. This microarray was used to analyze gene expression in neuroblastoma differentiation by RA from 1 to 8 days. The screened genes were identified and verified by semi-quantitative RT-PCR. Results: After analyzed, the data showed that there were 13 up-regulated genes, but no down-regulated gene. Conclusion: There are some diferentially expressed genes in the process of RA inducing SH-SY5Y to neuron. Our results indicate that the oligonucleotide microarray is reliable and can be used for dissecting molecular mechanism in the progress of neuroblastoma differentiation.