2008•Yixue yanjiusheng xuebaoRequires access
The effect of Atractyloside on sevoflurane postconditioning in focal cerebral ischemia
Lize Xiong
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Abstract
Objective: To investigate the effect of Atractyloside on sevoflurane postconditioning in focal cerebral ischemia.Methods: Fifty male SD rats were randomly assigned to five groups(n=10 each): control group(con),1.0 MAC group(sevo),1.0 MAC+ Atractyloside group(sevo+Atr),1.0 MAC+ vehicle group(sevo+vehicle)and Atractyloside group(Atr).All animals were subjected to the right middle cerebral artery occlusion(MCAO) for 120 min followed by reperfusion for 72 h.The animals in sevo groups were given 1.0 MAC sevoflurane inhalation from 20 min before to 10 min after reperfusion.The animals in Atr groups were given Atractyloside by ICV injection before sevoflurane postcondioning.The neurological deficit scores(NDS) were recorded at 24 h,48 h,and 72 h after reperfusion.Infarct volume percentage was determined after the last NDS assessment.Results: The infarct volume percentage ratio of sevo group(0.32±0.05) was significantly lower than that of con group(0.55±0.07)(P0.05).The infarct volume percentage of Sevo +Atr group(0.57±0.06)and Atr group(0.56±0.05) were not significantly different from that of con group(P0.05).The infarct volume percentage ratio of sevo+Atr group and Atr group were significantly higher than that of Sevo group(P0.05).Sevo+vehicle group(0.35+0.06) was not significantly different from that of Sevo group(P0.05).NDS of 24 h,48 h and 72 h after reperfusion in sevo group and sevo+vehicle were significantly higher than that in Con group.NDS of 24 h,48 h and 72 h after reperfusion in sevo+Atr group and Atr were significantly lower than that in Sevo group.Conclusion: Atractyloside abolished the protective effects of sevoflurane postconditioning on focal cerebral ischemia-reperfusion injury.