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Optimization on Alcohol Extraction Technology of Radix Arnebiae by Orthogonal Design

WU Xue-yuan, Tcm Pharmacy

Open publisher page 2 citations

Abstract

OBJECTIVE:To optimize the extraction technology of naphthoquinone such as L-shikonin in Radix Arnebiae.METHODS:An L9(34)orthogonal experiment was performed to investigate the 3 factors(three levels for each factors were selected),i.e.the amount of ethanol,temperature and time of extraction with the quantity of L-shikonin and extracta sicca yield as indexes for evaluation.RESULTS:The temperature of extraction had significant impact on the extraction of the effective components in Radix Arnebiae.The best extraction technology was as follows:adding 12-fold and 8-fold ethanol solution(at a concentration of 95%)for digeratur extraction twice at 65℃(1.0 h each time).CONCLUSION:The optimized technology for the extraction of L-shikonin from Radix Arnebiae was stable,reasonable and feasible.

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OBJECTIVE:To optimize the extraction technology of naphthoquinone such as L-shikonin in Radix Arnebiae.METHODS:An L9(34)orthogonal experiment was performed to investigate the 3 factors(three levels for each factors were selected),i.e.the amount of ethanol,temperature and time of extraction with the quantity of L-shikonin and extracta sicca yield as indexes for evaluation.RESULTS:The temperature of extraction had significant impact on the extraction of the effective components in Radix Arnebiae.The best extraction technology was as follows:adding 12-fold and 8-fold ethanol solution(at a concentration of 95%)for digeratur extraction twice at 65℃(1.0 h each time).CONCLUSION:The optimized technology for the extraction of L-shikonin from Radix Arnebiae was stable,reasonable and feasible.

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Available abstract

OBJECTIVE:To optimize the extraction technology of naphthoquinone such as L-shikonin in Radix Arnebiae.METHODS:An L9(34)orthogonal experiment was performed to investigate the 3 factors(three levels for each factors were selected),i.e.the amount of ethanol,temperature and time of extraction with the quantity of L-shikonin and extracta sicca yield as indexes for evaluation.RESULTS:The temperature of extraction had significant impact on the extraction of the effective components in Radix Arnebiae.The best extraction technology was as follows:adding 12-fold and 8-fold ethanol solution(at a concentration of 95%)for digeratur extraction twice at 65℃(1.0 h each time).CONCLUSION:The optimized technology for the extraction of L-shikonin from Radix Arnebiae was stable,reasonable and feasible.

Key concepts: Radix (gastropod), Extraction (chemistry), Chromatography, Yield (engineering), Alcohol, Mathematics, Chemistry, Process engineering

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