Calli transformation of maize inbred via Agrobacterium

Embryogenic calli of elite maize inbred Qi319, Ye515 and Ye502 were transformed via Agrobacterium tumefaciens , and plantlets were regenerated from resistant calli The concentration of Agrobacterium, duration of co-culture and the genotype of maize could affect the transformation efficiency of calli via Agrobacterium With the concentration of Agrobacterium at OD 600 0 5-0 7, and 3-4 days of co-culture, the transformation efficiency of calli was higher compared with other conditions Under identical conditions, the transformation efficiency of different genotypes of calli was 3 3%-10%