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2009•Journal of HydroecologyRequires access

Idntification of Edwarsiella tarda Strains by Two-Temperature PCR

Xiaoli Yu

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Abstract

A pair of primer XZE15 and XZE16 was designed and synthesized according to the conserved gene sequences of 23S rDNA of Edwarsiella tarda,and then reaction parameters were optimized to develop two-temperature polymerase chain reaction(PCR)to simultaneously identify E.tarda.The results showed that only of 284pb-long DNA fragment for three E.tarda strains was amplified,and no DNA fragments for three Edwarsiella ictalur strains and other fish pathogenic viruses and bacteria were amplified by this two-temperature PCR.And 10 pg DNA of E.tarda was detected by this two-temperature PCR.

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What this paper is about

A pair of primer XZE15 and XZE16 was designed and synthesized according to the conserved gene sequences of 23S rDNA of Edwarsiella tarda,and then reaction parameters were optimized to develop two-temperature polymerase chain reaction(PCR)to simultaneously identify E.tarda.The results showed that only of 284pb-long DNA fragment for three E.tarda strains was amplified,and no DNA fragments for three Edwarsiella ictalur strains and other fish pathogenic viruses and bacteria were amplified by this two-temperature PCR.And 10 pg DNA of E.tarda was detected by this two-temperature PCR.

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Available abstract

A pair of primer XZE15 and XZE16 was designed and synthesized according to the conserved gene sequences of 23S rDNA of Edwarsiella tarda,and then reaction parameters were optimized to develop two-temperature polymerase chain reaction(PCR)to simultaneously identify E.tarda.The results showed that only of 284pb-long DNA fragment for three E.tarda strains was amplified,and no DNA fragments for three Edwarsiella ictalur strains and other fish pathogenic viruses and bacteria were amplified by this two-temperature PCR.And 10 pg DNA of E.tarda was detected by this two-temperature PCR.

Key concepts: Primer (cosmetics), Polymerase chain reaction, Biology, DNA, Molecular biology, Gene, Bacteria, Virology

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