Measurement and analysis of unrelated umbilical cord blood transplantation

Objective To prepare the cord blood stem cell for providing the basis for effective and safe clinical application.Methods Maternal and cord blood samples of HIV,HBsAg,HCV,TP,CMV,endotoxin,mycoplasma,cytomegalovirus,pathogen were detected,and stem cells were isolated by purification kit in vitro.Then stem cells the number of mononuclear cells were counted after extraction.Dynamic of stem cells was detected by Taiwan phenol blue staining.CD34 stem cells and lymphocyte subsets were tested by flow cytometry.Results The number of nucleated cells was(5.4±1.09)×107 after extraction,plus the number of stem cells had positive correlation with cord blood volume.Dynamic rate of stem cells was(98.7±1.3)%.CD34+ cord blood stem cells was(1.55±0.67)%(n=19).There were fewer CD3 cells in cord blood.Total cells number of CD4 and CD8 was significantly higher than CD3.Cord blood CD4/CD8 selected umbilical cord blood transplantation was also less than 1.3,which caused mild symptom of GVHD.Conclusion Preparation of cord blood stem cell can accord with safe standard for cell therapy.