The Preparation of Super E.coli Competent Cell and the Establishment of Fast Plasmid Transformation into E.coli

The influence of different prepatations on the transformation efficiency of E.colicompetent cells was studied.The results showed that the efficiency of super E.coli competent cells was higher than common one.And the efficiency of super E.colicompetent cells increased 966.1% over the common one.The efficiency of super E.colicompetent cells was no significant differences compared with the kit.Storing in-80℃,it was better to store competent cells with 7%DMSO and the efficiency of competent cells stored with 7%DMSO was 1.1×107,it increased 69% over the 15% glycerol.Meanwhile the influence of different transformation method was studied.A fast plasmid transformation system was established.Compared with common method,the efficiency of fast plasmid transformation system was 4.95×107,and the transformation time was only 12.5% to the common method,it cost 15 min.