Effect of Different Soil Moistures on Total Phenolic Acid Composition Accumulation and Relevant Enzymatic Activity of Salvia Miltiorrhiza Lamina
Xiaolei Liu
Abstract
Xiaolei Liu
Abstract
This paper studies total phenolic acid composition accumulation and change rules of relevant enzymatic activity of salvia miltiorrhiza lamina under the condition of different soil moistures(35%,55% and 75% of field capacity) through pot experiment with Salvia miltiorrhiza Bunge as the material.The result shows that,in the later period of drought stress,the total phenolic acid composition accumulation of salvia miltiorrhiza lamina significantly increases.The total phenolic acid content is the highest when treated at 55%,followed by 35% and 75%.The total phenolic acid content treated at 55% is 1.50 times of that at 75%;that at 35% is 1.31 times of that at 75%.In the earlier stage of treatment,55% soil moisture can promote the increase of PAL enzymatic activity,while 55% and 35% soil moisture in the later period of treatment can promote the increase of TAT enzymatic activity,but drought stress doesn't have significant influence on C4H enzymatic activity.The accumulation of total phenolic acid composition of salvia miltiorrhiza lamina is related to PAL and C4H enzyme and its obvious relation with TAT is not found.This provides theoretical reference for the development of salvia miltiorrhiza lamina resources and standard plantation and irrigation of salvia miltiorrhiza and provides guidance for the quality control of salvia miltiorrhiza medicinal material.
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This paper studies total phenolic acid composition accumulation and change rules of relevant enzymatic activity of salvia miltiorrhiza lamina under the condition of different soil moistures(35%,55% and 75% of field capacity) through pot experiment with Salvia miltiorrhiza Bunge as the material.The result shows that,in the later period of drought stress,the total phenolic acid composition accumulation of salvia miltiorrhiza lamina significantly increases.The total phenolic acid content is the highest when treated at 55%,followed by 35% and 75%.The total phenolic acid content treated at 55% is 1.50 times of that at 75%;that at 35% is 1.31 times of that at 75%.In the earlier stage of treatment,55% soil moisture can promote the increase of PAL enzymatic activity,while 55% and 35% soil moisture in the later period of treatment can promote the increase of TAT enzymatic activity,but drought stress doesn't have significant influence on C4H enzymatic activity.The accumulation of total phenolic acid composition of salvia miltiorrhiza lamina is related to PAL and C4H enzyme and its obvious relation with TAT is not found.This provides theoretical reference for the development of salvia miltiorrhiza lamina resources and standard plantation and irrigation of salvia miltiorrhiza and provides guidance for the quality control of salvia miltiorrhiza medicinal material.
Key concepts: Salvia miltiorrhiza, Salvia, Chemistry, Lamina, Composition (language), Enzyme assay, Phenolic acid, Water content