2008•Shengwu jiagong guochengRequires access
Optimization of the expression of argE gene in E.coli
Ping Wei
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Abstract
N-acetylornithine deacetylase was expressed in recombinant bacteria BL21(DE3)-pET22b-argE.The location of the target protein was determined.The effects of induction temperature,type of inducer concentration of inducer,initial cell density,induction time on the activity expression of the target protein were studied.The results showed that both IPTG and lactose could be inducers,and the induction effect of lactose was better than that of IPTG.With the optimized active inducing condition of adding 15 g/L lactose at initiated OD600 of 0.46 and cultivating for 18 h at 20 ℃,the NAOase activity was increased by 168 folds from initial 1.68U/mL to 182.99U/mL.