Cloning and expression of extracellular domain of ULBP3

Objective To study the effect of ULBP3 on NK cell proliferation and activation, we manage to obtain the extracellular domain of ULBP3 protein at first. Methods The extracellular domain of ULBP3 gene fragment was isolated by RT-PCR from human osteosarcoma cell line Saos-2. After sequencing identification, it was cloned into the pET32a prokaryotic expression vector, and then the recombinant plasmid was transduced into E.Coli. BL21, target protein expression was induced by IPTG.Results The sequencing result showed that the nucleotide sequence of the DNA fragment obtained was totally identical with ULBP3 extracellular domain in GeneBank. The recombinant pET32a plasmid was constructed successfully and the extracellular domain of ULBP3 protein was expressed stably in the recombinant bacteria.Conclusion The extracellular domain of ULBP3 protein obtained can be used for further research.