The significance of p16 and Ki-67 proteins in the diagnosis of cervical intraepithelial neoplasia

Objective To study the p16 and Ki-67 proteins in cervical carcinoma and to explore their potential in the diagnosis of cervical lesions.Methods Immunohistochemical technique was used to detect p16 and Ki-67 expression in 20 cases of normal or inflammatory cervical lesions,22 cervical CIN Ⅰ,20 cervical CIN Ⅱ,20 cervical CIN Ⅲ,20 cervical glandular dysplasia,and 20 cervial carcinoma.Results The positive expression rates of p16 and Ki-67 proteins in normal cervical tissue were significantly lower than the cervical precancerous lesions and cervical carcinoma,the difference were significant(P0.01).Ki-67 protein expressions in normal cervical tissue and CIN Ⅰ tissues compared with no significant difference(P0.05);p16 positive expression of basal cell hyperplasia in cervical tissue was compared with CIN Ⅰ,there was significant differences(P0.05),while the Ki-67 protein with no significant difference(P0.05);p16 coloring strongly positive Among CIN Ⅰ,CIN Ⅱ,CIN Ⅲ,and cervical comparison,there were significant differences(P0.01).Conclusion p16 protein can be good identification of benign cervix and cervical intraepithelial neoplasia and cervical cancer;identify basal cell hyperplasia of cervical organization and CIN Ⅰ organizations,p16 can play a role of diagnosis.Application of the coloring strength of p16 can be a very a good distinction between low-grade cervical lesions(CIN Ⅰ) and cervical lesion height(CIN Ⅱ,CIN Ⅲ),which will help the judge and grade of CIN.Application of Ki-67 can be a good marker of cervical lesions and cervical cancer,a high degree,but can not mark the cervix benign lesions and low-grade cervical lesions,should be combined with p16 protein.