2005•Xi'nan nongye xuebaoRequires access
Studies on transformation of the Pinellia tenata Agglutinin (pta) gene into rice mediated by Agrobacterium
Zou Liangping, Dengfeng Qi, Shiquan Wang
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Abstract
The pCAMBIA1304 vector that carried multi-gene(hygromycin gene,pta gene,mGFP and GUS genes) was transferred into calli,which were derived from immature embryos of three rice varieties(Japonica rice No.9 of Zhonghua and Ribenqing and Indica rice 501) by Agrobacterium tumefaciens.These calli were cultured on selective media,which contained 65,45 and 35 mg/?L hygromycin,respectively,and the resistant calli were obtained.Ninety-two positive transgenic plants(forty-three No.9 of Zhonghua,forty-five Ribenqing and four 501) were identified by PCR from 415 T_0 regeneration plants.Southern blotting assay for these transgenic progenies indicated that the pta gene was successfully introduced and integrated into the genome of the receptors.The result of hygromycin resistance showed that the outer-gene can be stably inherited and expressed in transgenic plants.