2007Europe PMC (PubMed Central)Requires access

The Rapid Propagation Technology of Sorbus pohuashanensis by Axillary Bud Regeneration Way

Yanru Guo, Yaguang Zhan, Ji LiLi

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Abstract

In order to study the rapid propagation technology for Sorbus pohuashanensis stem segments with axillary buds were chosen as explants. The results showed that the best basic media was MS; axillary buds were induced successfully on MS basal media supplemented with 6-BA(2.5 mg·L-1) and 2.4-D(0.5 mg·L-1); the optimum medium for bud proliferation was MS+6-BA(1.600~2.100) mg·L-1+NAA(0.140~0.230)mg·L-1,13 shoots per explant could be obtained at average;the shoots grew strongly on MS+6-BA0.2 mg·L-1+IBA0.2 mg·L-1; And 1/2MS+IBA0.3 mg·L-1 was the best medium for shoot rooting,with the rooting rate at 90.20%,and the average root number of 5.96;Plantlets survival rate reached 95.55% when transplanted on the mixture(humus 3:peat soil 2:sand 1).

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What this paper is about

In order to study the rapid propagation technology for Sorbus pohuashanensis stem segments with axillary buds were chosen as explants. The results showed that the best basic media was MS; axillary buds were induced successfully on MS basal media supplemented with 6-BA(2.5 mg·L-1) and 2.4-D(0.5 mg·L-1); the optimum medium for bud proliferation was MS+6-BA(1.600~2.100) mg·L-1+NAA(0.140~0.230)mg·L-1,13 shoots per explant could be obtained at average;the shoots grew strongly on MS+6-BA0.2 mg·L-1+IBA0.2 mg·L-1; And 1/2MS+IBA0.3 mg·L-1 was the best medium for shoot rooting,with the rooting rate at 90.20%,and the average root number of 5.96;Plantlets survival rate reached 95.55% when transplanted on the mixture(humus 3:peat soil 2:sand 1).

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Available abstract

In order to study the rapid propagation technology for Sorbus pohuashanensis stem segments with axillary buds were chosen as explants. The results showed that the best basic media was MS; axillary buds were induced successfully on MS basal media supplemented with 6-BA(2.5 mg·L-1) and 2.4-D(0.5 mg·L-1); the optimum medium for bud proliferation was MS+6-BA(1.600~2.100) mg·L-1+NAA(0.140~0.230)mg·L-1,13 shoots per explant could be obtained at average;the shoots grew strongly on MS+6-BA0.2 mg·L-1+IBA0.2 mg·L-1; And 1/2MS+IBA0.3 mg·L-1 was the best medium for shoot rooting,with the rooting rate at 90.20%,and the average root number of 5.96;Plantlets survival rate reached 95.55% when transplanted on the mixture(humus 3:peat soil 2:sand 1).

Key concepts: Axillary bud, Shoot, Biology, Explant culture, Humus, Botany, Horticulture, Micropropagation

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