Studies on the Extraction of Polysaccharide from Pleurotus eryngii Fruit Body

Several factors of affecting extraction rate of polysaccharide from Plenrotus eryngii fruit body such as extraction temperature, extraction times and the solid-liquid ratio (W:V) were studied by uniform design test. The result showed that the extraction temperature was the primary factor to affect the polysaccharide extraction rate. The optimum extraction conditions were as follows: the ratio of sample to water(1:50),temperature (100℃) and time (240mi.). The extraction rate was 4.35% under the optimal extraction conditions. The crude polysaccharide was purified by ethanol sedimentation, dialysis, DE-52 chromatography. The purified Plenrotus eryngii polysaccharide was examined with ultraviolet spectral analysis without any protein and nuclear acid. the maximum absorbing peak was in 192 nm.