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2003•Chinese journal of integrated traditional and Western medicineRequires access

Development of Cellular Model of Rat HSC Activation Stimulated by LPS

Fu-Jing He

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Abstract

Objective: Development of cellular model 1 of HSC activation. Methods: Isolated rat HSC cultured in vitro was stimulated with lipopolysaccharide (LPS). Proliferation of rat HSC was detected with MTT technique. Expression levels of collagen I and collagen Ⅲ in rat HSC were showed with immunochemistry technique and analyzed with image analysis system. Results: LPS significantly promoted proliferation elevated expression levels of collagen I and collagen M in rat HSC. Conclusion: Model of rat HSC activation stimulated with LPS can reflect more intact HSC activation effects.

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Objective: Development of cellular model 1 of HSC activation. Methods: Isolated rat HSC cultured in vitro was stimulated with lipopolysaccharide (LPS). Proliferation of rat HSC was detected with MTT technique. Expression levels of collagen I and collagen Ⅲ in rat HSC were showed with immunochemistry technique and analyzed with image analysis system. Results: LPS significantly promoted proliferation elevated expression levels of collagen I and collagen M in rat HSC. Conclusion: Model of rat HSC activation stimulated with LPS can reflect more intact HSC activation effects.

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Available abstract

Objective: Development of cellular model 1 of HSC activation. Methods: Isolated rat HSC cultured in vitro was stimulated with lipopolysaccharide (LPS). Proliferation of rat HSC was detected with MTT technique. Expression levels of collagen I and collagen Ⅲ in rat HSC were showed with immunochemistry technique and analyzed with image analysis system. Results: LPS significantly promoted proliferation elevated expression levels of collagen I and collagen M in rat HSC. Conclusion: Model of rat HSC activation stimulated with LPS can reflect more intact HSC activation effects.

Key concepts: Immunochemistry, Lipopolysaccharide, In vitro, Cell biology, Chemistry, Molecular biology, Cell growth, Immunology

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