Effects of Total Flavones of Fructus Chorspondiati on Cell Cycle of Rat Cardiac Fibroblasts in vitro

Objective:To evaluate the effect of total flavones of fructus chorspondiatis(TFFC)on the cell cycle of cardiac fibroblasts(CFs)isolated from Sprague-Dawley rat induced by angiotensin II(Ang II),and its relationship with activity of NOS-NO(nitric oxygen syntheses-nitric oxygen)system. Methods: CFs were cultured in vitro and stimulated with 10-7 mol/L AngⅡ. The cell cycle was determined by flow cytometry(FCM). The activity of the actate dehydrogenase(LDH)was used to detect cardiac fibroblasts membrane permeability changes. The cellular growth and proliferation were detected by using trypan blue staining. Nitrate enzyme reverting method was also adopted to evaluate the NO content,and NOS activity was estimated by chemical colorimetric method. Results:Based on the analysis of FCM cell cycle,it showed that the percentage of cells on S and PI stage in CFs were gradually declined as TFFC concentration increased, while the percentage of cells on G0/G1 stage raised(P0.01). NO concentration in supernatant [(23.23± 0.70)μmol/L] treated by 100 mg/L TFFC for 72 hours,and was significantly higher than that of AngⅡ group[(20.20±0.83)μmol/L,P0.01]. NOS activity in supernatant[(20.43±0.53)U/L] treated by 100 mg/L TFFC for 72 hours,and was also significantly higher than that of AngⅡ group[(20.90±0.85)U/L, P0.01). There was a significant positive relevance between NO contents and NOS activity(r=0.964,P0.01). Conclusion:TFFC can inhibit the cell cycle proliferation of CFs in vitro,which maybe correlates with upregulation of NOS-NO system.