Effect of ginkgolide B on NF-κB expression in pulmonary tissue of mice with acute lung injury

Objective To investigate the effect of ginkgolide B(BN52021) on the NF-κB expression in pulmonary tissue of mice with LPS-induced acute lung injury.Methods Fifty Kunming mice were divided into 2 groups at random: LPS group(L group) and BN52021 pretreated group(B group),and then each group was subsequently divided into 5 subgroups according to different time point(0,1,3,9 and 12 h).The mice from corresponding groups received an intraperitoneal injection of normal saline or 20 mg/kg BN52021 followed by an injection of 15 mg/kg LPS 5 min later.The mice were sacrificed in 0,1,3,9 and 12 h after LPS injection.The wet/dry ratio(W/D) was recorded for pulmonary function and the pathological changes of lung tissues stained by HE were examined by light microscopy.The expression of NF-κB p65 was detected by Western blotting,and the levels of tumor necrosis factor-α(TNF-α) and interleukin-10(IL-10) were determined by ELISA.Results The significant increase of W/D was found after LPS injection in L group,which indicated the existence of pulmonary edema;while the W/D in B group was lower(P0.05 or P0.01).The pathological changes of inflammation,such as pulmonary hemorrhage and the inflammatory cells infiltration,were obvious in 2 groups,but that was better in B group.Moreover,the expression of NF-κB p65 displayed double peak at 1 h and 9 h in L group,while there was only one at 9 h in B group.What's more,the value in B group decreased in comparison with L group(P0.05) except for the value at 9 h.At last,the level of TNF-α in B group was lower than in L group,but there was no difference of IL-10 between 2 groups.Conclusion BN52021 can induce the inflammation of acute lung injury induced by LPS via inhibiting the early activation of NF-κB.