Isolation and tracing of side population from lentivirus-transduced GFP-positive ovarian cancer cells

Objective To testify whether lentivirus transduction has influence on the capability of ovarian cancer cells to pump out Hoechst 33342 and whether GFP can be used as a marker to trace cancer cell mobility in vivo.Methods HO-8910PM cells were transduced by lentivirus at various multiplicity of infection(MOI).Expression of transgene was clarified by FACs analysis.The best MOI under the control of Ubiquitin promoter was determined referring to GFP expression.GFP(+)HO-8910PM were collected by FACsorting and passaged under regular culture condition.GFP(+)SP cells were sorted after stained with Hoechst 33342 and injected subcutaneously into nude mice.The local tumorigenecity of GFP(+)SP cells at injection site was compared to that of non-transduced cells.The fluorescence images of tumor-bearing mice were taken under a small animal fluorescence imaging system. Results Lentiviral transduction under the control of Ubiquitin promoter had no effect on the capability of HO-8910PM cell to pump Hoechst 33342 out.GFP(+)SP cells from HO-8910PM could form GFP(+) tumor in nude mice.There was no difference in tumorigenecity between GFP(+)SP cells and non-transduced SP cells.Fluorescence in tumor cells at subcutaneous injection site could be detected under a fluorescence imaging system. Conclusions Lentiviral transduction has no affect on the capability of ovarian cancer SP cells-derived tumor formation.Lentiviral transduction can be a useful tool in the study of tumorigenesis and development of ovarian cancer.GFP can be a marker to trace cancer cells in vivo.