Construction of the Recombinant Eukaryotic Expression Vector of Coding Sequence of Porcine Myostatin

In this research,the coding sequence of MSTN mature protein was amplified by PCR from myostatin plasmid pMD18 T MSTN of a new porcine breed JunMu 1.The fragment with the expected size was cloned into vector pMD18 T and subsequently subjected to PCR,restriction endonucleases analysis and sequencing.Then,the fragment was cloned into vector pcDNA3 1 for eukaryotic expression.The coding sequcene of MSTN mature protein was coincidence with our design after PCR,restriction endonucleases analysis and sequencing.The expression of the cloned coding sequence of MSTN mature protein in eukaryotic system is under way.