Effects of TanshinonIIA on proliferation and apoptosis of hHuman cervical carcinoma Hela cells in vitro

[Objective] To investigate the regulatory effect of TanshinonⅡA on proliferation and apoptosis of human cervical carcinoma Hela cells in vitro.[Methods] Human cervical carcinoma cell line Hela was cultured in vitro.Hela cell were treated with 0.5～8.0 μg/L TanshinonⅡA for 24～72 h and the growth inhibition rate were measured with MTT method.Cell apoptosis was inspected by electron microscopy.apoptotic rate was quantified flow cytometry(FCM).[Results] After treatment with 0.5～8.0 μg/L TanshinonⅡA for 72 h the proliferation of Hela cells was significantly inhibited,the growth inhibition rate was 17.23%,24.27%,31.75%,39.37%,55.45%,respectively.Cell apoptosis occurred characterized by cell shrinkage.nuclear chromatin condensation and fragmentation.Formation of membrane blebs and apoptosis bodies as observed under fluorescence microscope and transmission electron microscope.The apoptosis rates quantified tanshinon ⅡA 0.5～8.0 μg/L for 72 h by flow cytometry(FCM) were 12.37%,25.81%,27.98%,34.13%,45.84%.[Cunclusion] Tanshinon ⅡA could significantly inhibit the growth of Hela cell.Tanshinon ⅡA can induce the apoptosis of human cervical carcinoma Hela cells in vitro,which may be related to the mechanism of growth inhibition of human cervical carcinoma Hela cells line.