IDENTIFICATION AND ANALYSIS OF H2O2 GENERATING LACTOBACILL IN VAGINA OF LACTOBACILLI PATIENTS WITH BACTERIAL VAGINOSIS

Objective:To identify and analyse H2O2 generating Lactobacillin in vagina of lactobacilli mongolian childbearing patients with bacterial vaginosis(BV)in pastoral area,so that to provide bases for the vaginal microcological treatment.Method:102 patients with BV and 98 mongolian healthy women were selected from 2008-06 to 2008-12 in inner mongolia pastoral area.PH value was detected in vagina,and vaginal secretions were examined through routine microscopic examination.Lactobacillus were isolated and cultured followed by the extraction of total microbial community DNA for identificate gene sequencing of 16S rDNA.Result:22 out of 102 BV cases were found out Lactobacillus,the detected rate was 21.6%,(74/98) women was detected Lactobacillus among 98 Mongolian healthy women.It showed significant difference between two groups(P=0.001).The isolation rate of H2O2 generating Lactobacilli was 27.3%(6/22)in all of BVpatients.75.7%(56/74)of Mongolian healthy women were found out H2O2 generating Lactobacill.The dominant species of lactobacilli in the vagina of the BV patients and mongolian healthy women were L.crispatus,L.acidophilus,L.gasseri,L.casei and L.jensenii.Conclusion:The amount of lactobacilli and H2O2 generating Lactobacilli in the vagina of BV patients was markbly less than that of childbearing age and healthy women.