Characteristics of Protease Produced by Microbes in Fermentation Bed for Ecological Pig-raising

The activities of proteases that produced by fermented B acillus Wuhan,Bacillus subtilis,Bacillus 7th,Bacillus 9th,Bacillus Y2 and BB1 Bacillus were determined by Folin method.The results showed that proteases produced by Bacillus 7th,Bacillus 9th and Bacillus Y2 had lower activities,but proteases produced by Bacillus Wuhan,Bacillus subtilis and BB1 Bacillus had much higher activities.Then,add trace elements to the three strains' mediums that with high producing protease activity to optimize conditions of enzyme production.The results indicated that it had a large impact on the protease production of Bacillus Wuhan,but it had a small impact on the protease production of Bacillus subtilis and BB1 Bacillus.