Clinical significance of detecting serum hepatitis B virus large surface protein in preoperative screening in patients with HBeAg-negative hepatitis B infection

Objective To detect serum hepatitis B virus large surface protein (HBV-LP) in preoperative screening in subjects with normal liver function and patients with HBeAg-negative hepatitis B infection, and to evaluate the significance of serum HBV-LP in the patients with hepatitis B. Methods HBV-LP, hepatitis B S1 (HBVpreS1) were measured by ELISA, and HBV DNA levels were detected by fluorescence quantitative PCR in 180 HBeAg-negative HBV patients in preoperative screening. Results In the 180 patients, the positive rates of PreS1-Ag, HBV-LP and HBV DNA were 21.1%, 34.4% and 32.7%, respectively. χ2 test showed that the positive rates of three groups had significant difference (χ2 =9.145 7, P=0.010 3). Scheffe test showed that the positive rate of PreS1-Ag detection was significantly lower than that of HBV-LP and HBV DNA (P0.05), while no significant difference was found in the positive rates of HBV-LP and HBV DNA detection (P0.05). HBV-LP and HBV DNA showed no significant differences in detection results (χ2 =0.173 9, P=0.676 7). At the same time, correlation analysis showed a good correlation between HBV-LP and HBV DNA detection (χ2 =95.655, P=0.0000, r=0.721 7). The scatter plot of 49 cases of HBV DNA copy number per ml (copies/ml) commonly used numerical and quantitative HBV-LP OD showed a positive correlation (r=0.6192). Conclusion HBV-LP, superior to HBVpreS1, is a good indicator in response to viral replication. There was a good correlation between HBV-LP and HBV-DNA detection, which can be used as a substitute and supplement of hepatitis B infection in hospital.