Effects of Antagonistic Bacteria B9 on Botrytis cinerea and Colonization on the Leaves of Tomato

The methods of mycelial growing rate,inhibitory zones and spore germination were used to examin the effects of B9 strain on Botrytis cinerea.The results indicated B9 strain showed obvious and stable inhibition activity against the mycelial growth and conidiospore germination of Botrytis cinerea.The original concentration of the bacteria all had the highest inhibition ratio-over 80%.The colonization ability of B9 strain on the leaves of tomato plants was also studied.The results showed that after introduing the bacteria 3 days,the antagonistic bacteria maintained stable high levels on the leaf,then the colonization quantities decreased rapidly,but still lasted for about 20 days.Temperature and inoculation of Botrytis cinerea all had a great effect on colonization of the antagonists.While in the condition of higher temperature(about 28℃) and pre-inoculation of B9 strain one day,the antagonistic bacteria showed the strongest ability of colonization on the leaf,which had the biggest colonization quantities.