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Effects of VE deficiency on activity of Caspase-3 and expression of Caspase-3 mRNA of splenic lymphocytes in chicken

Jiawang Feng

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Abstract

The activity of Caspase-3 and expression of Caspase-3 mRNA of splenic lymphocytes in the chicken with Vitamin E(VE) deficiency were detected by colorimetry and semi-quantitative reverse transcriptase-polymerase chain reaction(RT-PCR).The results showed that the activity of Caspase-3 and abundance of Caspase-3 mRNA was higher in VE deficiency chicken than those of control(P0.01).The results indicate that the changes of Caspase-3 activity and Caspase-3 mRNA abundance are one of the regulation mechanisms on lymphocyte apoptosis in the chicken with VE deficiency.

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What this paper is about

The activity of Caspase-3 and expression of Caspase-3 mRNA of splenic lymphocytes in the chicken with Vitamin E(VE) deficiency were detected by colorimetry and semi-quantitative reverse transcriptase-polymerase chain reaction(RT-PCR).The results showed that the activity of Caspase-3 and abundance of Caspase-3 mRNA was higher in VE deficiency chicken than those of control(P0.01).The results indicate that the changes of Caspase-3 activity and Caspase-3 mRNA abundance are one of the regulation mechanisms on lymphocyte apoptosis in the chicken with VE deficiency.

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Available abstract

The activity of Caspase-3 and expression of Caspase-3 mRNA of splenic lymphocytes in the chicken with Vitamin E(VE) deficiency were detected by colorimetry and semi-quantitative reverse transcriptase-polymerase chain reaction(RT-PCR).The results showed that the activity of Caspase-3 and abundance of Caspase-3 mRNA was higher in VE deficiency chicken than those of control(P0.01).The results indicate that the changes of Caspase-3 activity and Caspase-3 mRNA abundance are one of the regulation mechanisms on lymphocyte apoptosis in the chicken with VE deficiency.

Key concepts: Biology, Messenger RNA, Caspase 2, Caspase 3, Molecular biology, Apoptosis, Caspase, Reverse transcriptase

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Effects of VE deficiency on activity of Caspase-3 and expression of Caspase-3 mRNA of splenic lymphocytes in chicken — Research Paper | ScholarLens