The Effect of Dexamethasone on Expression of Aquaporin-1 in Cultured Human Trabecular Meshwork Cells

OBJECTIVE:To study the effect of dexamethasone (Dex) on expression of aquaporin-1(AQP1) in cultured human trabecular meshwork(HTM) cells METHODS:Reverse transcription combined with polymerase chain reaction(RT-PCR) was used to detect the expression of AQP1 in cultured HTM cells and those treated by Dex RESULTS:The mRNA of AQP1 expressed in normal HTM cells was 1 643±0 354,while 1 577±0 405,1 117±0 443,0 458±0 301,0 267±0 243 in those treated with Dex for 3 days in concentrations of 10-8mol,5×10-8 mol,10-7 mol,5×10-7mol As the concentrations of Dex increased to≥5×10-8mol,the expression of AQP1 was inhibited(P0 05) CONCLUSION:The expression of aquaporin-1 is inhibited by Dex in HTM cells It may be one reason that causes the reduction of aqueous outflow in corticosteroid induced glaucoma