Effect of endostatin combined with adriamycin on the proliferation and apoptosis of human glioma sarcomatosum cell line GL15 cells

Objective To investigate the effect of endostatin(ES) or ES combined with adriamycin(ADM) on the proliferation and apoptosis of human glioma sarcomatosum cell line GL15 cells.Methods The pcDNA 3.1 ES was transfected into GL15 cells.The effects of ES or ES+ADM on proliferation and apoprosis of GL15 cells were detected by MTT and flow cytometry.Results The inhibition of ADM to growth of the GL15 cells transfected with pcDNA 3.1 ES was enhanced significantly,and proliferation was reduced significantly by MTT(P0.05).The flow cytometry revealed that after transfection of pcDNA 3.1 ES into GL15 cells,the G0/G1 stage cell rate of GL15 cells line was increased significantly(P0.05),the S stage cell rate was significantly reduced and apoptosis rate significantly increased(P0.01).After combined use of ES and ADM,all the changes were more significant(P0.01).Conclusion The combination of ES and ADM could inhibit the growth of human glioma sarcomatosum cell line GL15 cells synergically.