The Influence of the deficiency of kidney and spleen-Yang on Klotho gene expression in model mice

Objective Using injected with hydrocortisone and administered with decoction liquid of Rhubarb had established deficiency of kidney and spleen-Yang disease models to observe the hippocampus changes and the influence on Klotho gene expression. Methods Totally 40 SD male mice at the age of eight weeks,were randomly divided into normal control group,model group,Vitamin E group and Guifulizhongwan group,with ten normal mice in each group,which were separately given medicine. Excluding normal control group,the mice were injected with hydrocortisone and administered with decoction liquid of Rhubarb for established deficiency of kidney and spleen-Yang disease models,the procedures lasted for 30 days. Observation the ultrastructure of hippocampus changes; detection of Klotho mRNA expression by real-time fluorescence quantitative polymerase chain reaction( PCR) method; adopting protein immune imprinting( Western blotting) method and immune histochemistry staining method to detection of klotho protein expressions. Results Compared with the normal control group,the model group mice which of hippocampal organization organelles were reduced,the neuron atrophy,neurons synaptic gap widening; compared with the model control group,the Vitamin E and Guifulizhongwan group mice which of hippocampal organization organelles were increased,withers as rarely as the neuron atrophy,neurons synaptic gap widening. Compared with the normal control group,the model group mice Klotho expression had obviously decreased( P 0. 05); compared with the model control group,the Guifulizhongwan group mice whice of Klotho expression levels had obviously increased( P 0. 05). Conclusion The deficiency of kidney and spleen-Yang disease models mice of the brain hippocampus of Klotho expression levels were decreased,resulting in ability to clear the oxygen free radical was reduced,increasing in superoxide damage to the body,to speed up the body's aging. The recipe of warming and tonifying the spleen and kidney-Guifulizhongwan can increased expression of Klotho expression levels,prompted an increase in antioxidant enzyme synthesis,repaired organism damage organization organ,thus the postponement deficiency of kidney and spleen-Yan causes the aging advancement.