Study on extraction of soybean protein in saponified P_(204) microemulsion system

The mechanism and technics of extracting soybean protein was studies in microemulsion system of NaOH,di(2-ethylhexyl) phosphoric acid(P204) and octane.The effect factors on extracting soybean protein from aqueous phase in this microemulsion system,including the mass of soybean,the concentration of P204 and NaOH,the extraction time,the pH in the external aqueous phase and the intensity of ion,were investigated.The experimental results show that the optimum extraction conditions are as following: the mass/volume rate of soybean to micriemulsion was 1:10,the concentration of P204 in oil phase was 0.8 mol/L,the concentration of NaOH was1.25 mol/L,the extraction time was 15 min,the pH in the external aqueous phase was 5,the highest extraction efficiency of the protein was 88.48 %.