Preparation of reducing sugars with synergistic hydrolysis of microwave irradiation and cellulase from poplar
Gong Zheng-wu
Abstract
Gong Zheng-wu
Abstract
Reducing sugars was made from poplar powder with synergistic hydrolysis of microwave irradiation and cellulase. The effects of sample size,amount of cellulase,reaction time and reaction temperature on the yield of reducing sugars were investigated. The condition of temperatures,times and amounts of cellulase were analyzed by response surface methodology(RSM),and the best processes were obtained. The result showed the yield of the reducing sugars was 32.40% as the reaction temperature was 50.5℃,the time was 20.7h and the amount of cellulase was 27U/g. The yield had been increased 11.87% compared with the sample which did not have microwave irradiation at the same condition.
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Reducing sugars was made from poplar powder with synergistic hydrolysis of microwave irradiation and cellulase. The effects of sample size,amount of cellulase,reaction time and reaction temperature on the yield of reducing sugars were investigated. The condition of temperatures,times and amounts of cellulase were analyzed by response surface methodology(RSM),and the best processes were obtained. The result showed the yield of the reducing sugars was 32.40% as the reaction temperature was 50.5℃,the time was 20.7h and the amount of cellulase was 27U/g. The yield had been increased 11.87% compared with the sample which did not have microwave irradiation at the same condition.
Key concepts: Cellulase, Hydrolysis, Yield (engineering), Chemistry, Response surface methodology, Reducing sugar, Enzymatic hydrolysis, Microwave irradiation