The Hydrolysis of Oyster Meat with Two Kinds of Proteinase and Using HCl

Oyster meat was hydrolyzed to prepare amino acid hydrolysate using neutral protease and pineapple protease simultaneously and using HCl,respectively.The results showed that the optimum conditions of enzymatic hydrolysis were as follows: the temperature was 50 ℃,pH was 7.0,the amount of enzyme was 2.3×10-5 Kat/g,expects the hydraulic modulus was 1∶8,the time was 4 h.After 4 h hydrolysis of neutral,pineapple protease were used by step for 4 h respectively.The result indicated that seventeen kinds of amino acids were detected,which included seven essential amino acids in humans.