Detection Antibiotic Resistance,SCCmec typing and toxic genes of Methicillin-Resistant Staphylococcus aureus

Objective To investigate the genotype of Staphylococcal chromosomal cassette mec(SCCmec),and antibiotic resistance,panton-valentine leukocidin gene(PVL) and Toxic Shock Syndrome Toxin 1 Gene(TSST-1) of clinical methicillin-resistant Staphylococcus aureus(MRSA) isolates in Peking University First Hospital,preliminarily find out multi-drug resistance reason and provide molecular epidemiological evidence for strategies to control MRSA infection.Methods 53 isolates of nosogenetic MRSA were collected from Sep 2007 to Mar 2008.The antimicrobial susceptibility test was proceeded by automated Microbiology Analyzer,SCCmec typing was determined by multiplex PCR,PVL and TSST-1 were confirmed by PCR.Results 53 isolates of MRSA are multiresistant,SCCmec typing show that most isolates are ⅢA type(47.2%),PVL gene in 53 MRSA isolates are all negative,the rate of TSST-1+ is 22%.Conclusion The multiresistant characteristic of 53 MRSA isolates closely connect with SCCmec typing,SCCmec typing is a research mean for exploring the relationship between the structure and the mechanism of multi-drug resistance,TSST-1 gene positive strains were in a high proportion in the clinical isolates of S.aureus.