Studies on Tissue Culture and Rapid Propagation of Pulsatilla koreana Nakai

Leaf,petiole and pedicle explants of Pulsatilla koreana were cultured on MS medium supplemented with various concentrations of zeatin,kinetin or 6-BA combined with 0.05 mg·L-1 IAA.After 6 weeks of culture,effects of cytokinin on adventitious shoot formation from explants were investigated.The highest frequency of shoot formation was obtained when petiole and pedicle explants were cultured on MS+0.5 mg·L-1 zeatin+0.05 mg·L-1 IAA.Regenerated shoot were cut as several cluster or single plant with callus like tissue,and then transferred on MS+0.5 mg·L-1 zeatin+0.05mg·L-1 IAA or MS+1.0 mg·L-1 6-BA+0.05 mg·L-1 IAA for shoot propagation and growth.When shoot reached more than 3 cm in length,they were transferred on to root induction medium.93 percent of rooting ratio was observed in 1/2 MS+1.5 mg·L-1 NAA,after 42 days of culture.The rooted plantlets were acclimatized on mixture of North east mountain soil and perlite(2:1).