Optimized Polyethylenimine as a Non-viral Vector for DNA Transfection

Objective The purpose of this study is to investigate the optimal conditions for DNA transfection with polyethylenimine (PEI).Methods The influences of various factors on the transfection efficiency of 25 kDa linear PEI were investigated to get optimal conditions.Results PEI could fully condense DNA with a PEL/DNA ratio≥2,and the optimal mixing time was 10 mi- nutes.But the presence of serum significantly lowered the efficiency of PEI.The increasing PEI/ DNA ratio could raise transfection efficiency.The optimal density of 293T cells for transfection was 80%.Conclusion The factors affecting the transfection efficiency of PEI were studied,and the optimal conditions were identified.