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2008•The Chinese Journal of DermatovenereologyRequires access

Apoptosis of Keratinocyte Induced by Curcumin and Its Mechanism

Xiao Jun-gang

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Abstract

Objective To investigate the influence of curcumin on the apoptosis of keratinocyte and its mechanism of action in vitro.Methods The effects of curcumin of consecutive concentrations on the proliferation of HaCaT were observed by MTT assay.Apoptosis was evaluated by flow cytometry.The activity of caspase-3 and caspase-9 was determined by fluorescent analysis.Results After HaCaT were treated with curcumin(5umol/L),the percentage of the early apoptotic cells were significantly increased and the levels of Caspase-3 and caspase-9 activity were increased after the treatment.Conclusion Curcumin induces apoptosis of HaCaT cells through activation of caspase-9 and caspase-3.

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What this paper is about

Objective To investigate the influence of curcumin on the apoptosis of keratinocyte and its mechanism of action in vitro.Methods The effects of curcumin of consecutive concentrations on the proliferation of HaCaT were observed by MTT assay.Apoptosis was evaluated by flow cytometry.The activity of caspase-3 and caspase-9 was determined by fluorescent analysis.Results After HaCaT were treated with curcumin(5umol/L),the percentage of the early apoptotic cells were significantly increased and the levels of Caspase-3 and caspase-9 activity were increased after the treatment.Conclusion Curcumin induces apoptosis of HaCaT cells through activation of caspase-9 and caspase-3.

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Available abstract

Objective To investigate the influence of curcumin on the apoptosis of keratinocyte and its mechanism of action in vitro.Methods The effects of curcumin of consecutive concentrations on the proliferation of HaCaT were observed by MTT assay.Apoptosis was evaluated by flow cytometry.The activity of caspase-3 and caspase-9 was determined by fluorescent analysis.Results After HaCaT were treated with curcumin(5umol/L),the percentage of the early apoptotic cells were significantly increased and the levels of Caspase-3 and caspase-9 activity were increased after the treatment.Conclusion Curcumin induces apoptosis of HaCaT cells through activation of caspase-9 and caspase-3.

Key concepts: HaCaT, Curcumin, Apoptosis, Keratinocyte, Flow cytometry, Chemistry, In vitro, MTT assay

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