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2007•The Practical Journal of CancerRequires access

The Clinical Significance of Cytogenetics and FISH in the Diagnosis of Acute Promyelocyte Leukemia

Jianbo Cheng

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Abstract

Objective To investigate the clinical significance of cytogenetic study and FISH in the diagnosis of APL.Methods G binding technique for the presence of t (15; 17) translocation and FISH for PML-PARα fusion genes were used.Results Of the 38 patients diagnosed finally as APL, 36 patients(92.1%) were diagnosed as APL based on morphologic feature, 36 patients (92.1%) had clonal t (15; 17) change in cytogenetic study and 38(100%) patients had the PML-RARα fusion gene detected by FISH study.Conclusion Both cytogenetic study and FISH study for t(15;17)associated PML-RARα fusion gene are reliable in APL diagnosis, and especially, FISH study is more sensitive than cytogenetic study.

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What this paper is about

Objective To investigate the clinical significance of cytogenetic study and FISH in the diagnosis of APL.Methods G binding technique for the presence of t (15; 17) translocation and FISH for PML-PARα fusion genes were used.Results Of the 38 patients diagnosed finally as APL, 36 patients(92.1%) were diagnosed as APL based on morphologic feature, 36 patients (92.1%) had clonal t (15; 17) change in cytogenetic study and 38(100%) patients had the PML-RARα fusion gene detected by FISH study.Conclusion Both cytogenetic study and FISH study for t(15;17)associated PML-RARα fusion gene are reliable in APL diagnosis, and especially, FISH study is more sensitive than cytogenetic study.

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Available abstract

Objective To investigate the clinical significance of cytogenetic study and FISH in the diagnosis of APL.Methods G binding technique for the presence of t (15; 17) translocation and FISH for PML-PARα fusion genes were used.Results Of the 38 patients diagnosed finally as APL, 36 patients(92.1%) were diagnosed as APL based on morphologic feature, 36 patients (92.1%) had clonal t (15; 17) change in cytogenetic study and 38(100%) patients had the PML-RARα fusion gene detected by FISH study.Conclusion Both cytogenetic study and FISH study for t(15;17)associated PML-RARα fusion gene are reliable in APL diagnosis, and especially, FISH study is more sensitive than cytogenetic study.

Key concepts: Promyelocyte, Fish <Actinopterygii>, Cytogenetics, Fusion gene, Chromosomal translocation, Biology, Leukemia, Karyotype

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