ScholarLens Back to search
2009•Acta Agriculturae Boreali-SinicaRequires access

Effect of the Different Buffer on Electrophoretic Mobility Shift Assay(EMSA)

Feng Zhi-fu

Open publisher page 0 citations

Abstract

The test of Electrophoretic Mobility Shift Assay is very simple in theory.But to successfully carry out gel shift experiments,it needs to optimize the parameters.Through a combination of T7 RNA polymerase and T7 Promoter for testing proper buffer was selected.The experimental results showed 5×binding-Buffer 1 was more suitable for the test;TBE running-buffer was better than TAE and TGE buffer;0.2×TBE buffer was the best concentration amang there electrophoresis buffer.

About this research paper

What this paper is about

The test of Electrophoretic Mobility Shift Assay is very simple in theory.But to successfully carry out gel shift experiments,it needs to optimize the parameters.Through a combination of T7 RNA polymerase and T7 Promoter for testing proper buffer was selected.The experimental results showed 5×binding-Buffer 1 was more suitable for the test;TBE running-buffer was better than TAE and TGE buffer;0.2×TBE buffer was the best concentration amang there electrophoresis buffer.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

The test of Electrophoretic Mobility Shift Assay is very simple in theory.But to successfully carry out gel shift experiments,it needs to optimize the parameters.Through a combination of T7 RNA polymerase and T7 Promoter for testing proper buffer was selected.The experimental results showed 5×binding-Buffer 1 was more suitable for the test;TBE running-buffer was better than TAE and TGE buffer;0.2×TBE buffer was the best concentration amang there electrophoresis buffer.

Key concepts: Buffer (optical fiber), Electrophoretic mobility shift assay, Electrophoresis, Chemistry, Chromatography, Buffer solution, Computer science, Biochemistry

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Effect of the Different Buffer on Electrophoretic Mobility Shift Assay(EMSA) — Research Paper | ScholarLens