Study on the mechanism of Mimeng Flower Decoction on VEGF signal transmission and inhibition of human umbilical vascular endothelial cells induced by chronic hypoxia

OBJECTIVE To study the mechanism of Mimeng Flower Decoction on VEGF signal transmission to inhibit the proliferation of human umbilical vascular endothelial cells(HUVEC) induced by chronic hypoxia.METHODS HUVEC were cultured in vitro.Used CoCl2 to set up the cell chemistry hypoxia model,in the same time,10,20,40mg/ml Mimeng Flower Decoction extract was added to the HUVEC separately,the following studies were made after incubation of 24h.Through RT-PCR method,detected the mRNA phosphorylation expression level of VEGF,Flt-1,Flk-1/KDR in HUVEC regulated by Mimeng Flower Decoction.RESULTS The RT-PCR test showed that the expression of VEGFmRNA,Flk-1/KDRmRNA increased while Flt-1mRNA reduced in hypoxia group compared with blank group(p0.01).Mimeng Flower Decoction could down regulate the expression of VEGFmRNA,Flk-1/KDRmRNA and up regulate the expression of Flt-1mRNA compared with hypoxia group(p0.01) and these effects were dose dependent.CONCLUSIONS Mimeng Flower Decoction could markedly inhibit the HUVEC proliferation in hypoxia state.Down regulating the mRNA expression of VEGF,Flk-1/KDR mRNA and up regulating mRNA expression of Flt-1 mRNA may play a significant role in inhibiting proliferation of HUVEC by Mimeng Flower decoction.