Changes of nuclear factor-κB(NF-κB) in cultured bone marrow stromal cells after 60 Co γ-irradiation

Objective To explore the changes in bone marrow hematopoietic microenvironment after exposure to γ-radiation. Methods Immunohistochemistry,Western blot and electrophoretic mobility shift assay (EMSA)was used. Results The nuclear translocation was found in cultured bone marrow stromal cells after 60 Coγ-irradiation with immunohistochemistry.The expression of NF- κB in the bone marrow stromal cells (BMSCs) on the level of protein was elevated after exposure to 60 Coγ-rays at the dose of 8 0Gy with the uses of immunohistochemistry and Western blot.The activities of NF-κB in the BMSCs increased significantly after exposure to γ-radiation by using EMSA.The activity peak was at 4 hours after irradiation. Conclusion Our results suggest that the activation of NF-κB in the BMSCs by irradiation might be involved in the protection of BMSCs and in the recovery of hematopoiesis after γ-irradiation.\;